MagNA Lyser Green Beads from Roche

Product Specs
ItemMagNA Lyser Green Beads
CompanyRoche
Price Supplier Page View Company Product Page
Catalog Number03358941001
Quantity100 tubes (prefilled with ceramic beads)

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Characterization of a fluorescence imaging probe that exploits metabolic dependency of ovarian clear cell carcinomaScientific ReportsNovember 20, 2023Saki Tsuchimochi, Osamu Wada-Hiraike, Yasuteru Urano, Asako Kukita, Kohei Yamaguchi, Harunori Honjo, Ayumi Taguchi, Michihiro Tanikawa, Kenbun Sone, Mayuyo Mori-Uchino, et al.(Roche Diagnostics, Tokyo, Japan; Cat# 03358968001) and MagNA Lyser Green Beads (Roche Diagnostics; Cat# 03358941001). Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany; Cat# 74104), and cDNA

(2) Short salsalate administration affects cell proliferation, metabolism, and inflammation in polycystic kidney diseaseiScienceNovember 17, 2023Kanhai AA, Sánchez-López E, Kuipers TB, van Klinken JB, Dijkstra KL, van der Veen I, Baelde HJ, Song X, Pei Y, Mei H, et al.pellets containing salsalate,Special Diet Services,N/A MagNA Lyser Green Beads tubes,Roche Life Science,03358941001 Oligo-dT magnetic beads,New England BioLabs,S1419S 4–20% Criterion™ TGX™ Precast Midi Protein Gel,Bio-Rad Laboratories

(3) Next Generation Exon 51 Skipping Antisense Oligonucleotides for Duchenne Muscular DystrophyNucleic Acid TherapeuticsJune 1, 2023Judith van Deutekom, Chantal Beekman, Suzanne Bijl, Sieto Bosgra, Rani van den Eijnde, Dennis Franken, Bas Groenendaal, Bouchra Harquouli, Anneke Janson, Paul Koevoets, et al.(2–4 cycles of 20 s at 7,000 rpm and cooling on ice after 2 cycles) using MagNA Lyser Green Beads (No. 03358941001; Roche). The RNA concentration was determined with Nanodrop. cDNA was generated with 1,000 ng total

(4) RP11‐367G18.1 V2 enhances clear cell renal cell carcinoma progression via induction of epithelial–mesenchymal transitionCancer MedicineApril 1, 2023I‐Hung Shao, Pei‐Hua Peng, Heng‐Hsiung Wu, Ji‐Lin Chen, Joseph Chieh‐Yu Lai, Jeng‐Shou Chang, Han‐Tsang Wu, Kou‐Juey Wu, See‐Tong Pang, Kai‐Wen Hsufinal. 2.2 RNA sequencing (RNA‐seq) The homogenized sample was collapsed by MagNA Lyser Green Beads (03358941001, Roche) with frozen tissue in RLT. RNA was purified by RNeasy followed the protocol of manual factory.

(5) The Histone Methyltransferase SETD8 Regulates the Expression of Tumor Suppressor Genes via H4K20 Methylation and the p53 Signaling Pathway in Endometrial Cancer CellsCancers (Basel)October 31, 2022Asako Kukita, Kenbun Sone, Syuzo Kaneko, Eiryo Kawakami, Shinya Oki, Machiko Kojima, Miku Wada, Yusuke Toyohara, Yu Takahashi, Futaba Inoue, et al.MagNA Lyser Instrument (Roche, Basel, Switzerland; 03358968001) and MagNA Lyser Green Beads (Roche; 03358941001). Total RNA from freshly frozen tissues and endometrioid adenocarcinoma cells was isolated using the

(6) Chimeric immune checkpoint protein vaccines inhibit the tumorigenesis and growth of rat cholangiocarcinomaFrontiers in ImmunologyOctober 20, 2022Yi-Ru Pan, Chiao-En Wu, Wen-Kuan Huang, Ming-Huang Chen, Keng-Hsueh Lan, Chun-Nan YehRT-PCR Tumor samples were homogenized using MagNA Lyser Instrument and MagNA Lyser Green Beads (Roche 03358941001). RNA was extracted using Trizol and then was used for reverse-transcription with HiScript I TM First Strand cDNA Synthesis Kit

(7) Host and microbiome features of secondary infections in lethal covid-19iScienceSeptember 16, 2022Zacharias M, Kashofer K, Wurm P, Regitnig P, Schütte M, Neger M, Ehmann S, Marsh LM, Kwapiszewska G, Loibner M, et al.assays,Critical commercial assays,Critical commercial assays ,, MagNA Lyser green beads tubes,Roche,Cat# 03358941001 Maxwell 16 LEV simplyRNA blood kit,Promega,Cat# AS1310 QIAamp Viral RNA Mini Kit,Qiagen,Cat# 221413 High-Capacity

(8) Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transportersParticle and Fibre ToxicologyAugust 3, 2022Tereza Smutná et al.°C. The tissue samples with additional SDS lysis buffer were homogenized by MagNA Lyser Green Beads (03358941001, Roche, Prague, Czech Republic) using homogenizer. The samples were obtained as supernatant after centrifugation. Protein

(9) Pirfenidone increases IL-10 and improves acute pancreatitis in multiple clinically relevant murine modelsJCI insightJanuary 25, 2022Palathingal Bava E, George J, Tarique M, Iyer S, Sahay P, Gomez Aguilar B, Edwards DB, Giri B, Sethi V, Jain T, et al.quantitative PCR (qPCR). Pancreatic tissue was homogenized using MagNa Lyser tubes (Roche, catalog 03358941001) in TRIzol reagent (Thermo Fisher Scientific, catalog 15596026) and RNA was isolated using Qiagen RNEasy

(10) Transcription- and phosphorylation-dependent control of a functional interplay between XBP1s and PINK1 governs mitophagy and potentially impacts Parkinson disease pathophysiologyAutophagyDecember 1, 2021El Manaa W, Duplan E, Goiran T, Lauritzen I, Vaillant Beuchot L, Lacas-Gervais S, Morais VA, You H, Qi L, Salazar M, et al.brains of 2–4 or 6–10-month-old were transferred to green bead tubes (MagNA Lyser Green beads; Roche, 03358941001) containing 1 ml of lysis buffer (10 mM, Tris-HCl, pH 7.5 supplemented with a protease inhibitors cocktail

(11) Low dystrophin variability between muscles and stable expression over time in Becker muscular dystrophy using capillary Western immunoassayScientific ReportsDecember 1, 2021Z. Koeks et al.tablet (Roche/Sigma 04693159001)/8 ml; 5% β-Mercaptoethanol) plus approximately 15 MagNA Lyser beads (#03358941001, Roche) were added. After briefly spinning down, the sections were then homogenized by 2–4 cycles (20

(12) Chromatin accessibility analysis identifies GSTM1 as a prognostic marker in human glioblastoma patientsClinical epigeneticsNovember 3, 2021Huang YC, Lai JC, Peng PH, Wei KC, Wu KJ.extraction from frozen tissue and RNA-seq Frozen tissue was homogenized by MagNA Lyser Green Beads (03358941001, Roche). After homogenization, RNA was extracted by RNeasy according to the manufacturer’s protocol.

(13) TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cellsCell death & diseaseSeptember 23, 2021Woznicki JA, Saini N, Flood P, Rajaram S, Lee CM, Stamou P, Skowyra A, Bustamante-Garrido M, Regazzoni K, Crawford N, et al.RLT buffer with 1% β-mercaptoethanol and disrupted using MagNA Lyser instrument and Green Bead tubes (03358941001, Roche) with two homogenisation cycles of 15 sec at 6500 rpm. Biopsy homogenates were processed using

(14) DNAJB6b is Downregulated in SynucleinopathiesJournal of Parkinson's diseaseJanuary 1, 2021Folke J, Arkan S, Martinsson I, Aznar S, Gouras G, Brudek T, Hansen C.Life Technologies, 1:100). Brain tissue samples were homogenized using MagNA Lyser Green Beads tubes (cat# 03358941001, Roche Diagnostics) and homogenized twice on MagNA Lyser instrument (Roche Diagnostics, CH) for 25 s

(15) Detailed genetic and functional analysis of the hDMDdel52/mdx mouse modelPLOS ONEDecember 23, 2020Alper Yavas et al.β-mercaptoethanol). After briefly spinning down, approximately 25 ceramic beads (MagNA Lyser Green Beads; Roche 03358941001) were added to each tube. The samples were then homogenized by 2–4 cycles (20 seconds; 7000 rpm) in

(16) Molecular repertoire of Deinococcus radiodurans after 1 year of exposure outside the International Space Station within the Tanpopo missionMicrobiomeDecember 1, 2020Emanuel Ott, Yuko Kawaguchi, Denise Kölbl, Elke Rabbow, Petra Rettberg, Maximilian Mora, Christine Moissl-Eichinger, Wolfram Weckwerth, Akihiko Yamagishi, Tetyana Milojevic1000 μl QIAzol together with ceramic beads (Roche, Basel, Switzerland; MagNA Lyser Green Beads, CatNo 03358941001). Homogenization was performed as described above. After cell disruption, samples were incubated for

(17) Tissue‐specific deletion of mouse basolateral uniporter LAT4 (Slc43a2) reveals its crucial role in small intestine and kidney amino acid transportThe Journal of PhysiologyNovember 1, 2020Anuradha Rajendran, Nadège Poncet, Lalita Oparija‐Rogenmozere, Brigitte Herzog, François Verreycocktails. Homogenization was performed at 6000 rpm for 2 × 30 s with MagNa Lyser Green Beads (catalogue no. 03358941001; Roche Diagnostics) using the Precellys24 homogenizer (Precellys24; Bertin Instruments, Montigny‐le‐Bretonneux,

(18) Using a State-of-the-Art Toolbox to Evaluate Molecular and Functional Readouts of Antisense Oligonucleotide-Induced Exon Skipping in mdx MiceNucleic Acid TherapeuticsFebruary 1, 2020Nicole A. Datson et al.grinding in a MagNa Lyser (two to four cycles of 20 s at 7,000 rpm) using MagNA Lyser Green Beads (no. 03358941001; Roche). The RNA concentration was determined with Nanodrop. For cDNA synthesis, 1,000 ng of total RNA

(19) Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-γ and TNF-α in intestinal epithelial cellsCell death & diseaseJanuary 27, 2020Woznicki JA, Flood P, Bustamante-Garrido M, Stamou P, Moloney G, Fanning A, Zulquernain SA, McCarthy J, Shanahan F, Melgar S, et al.Sigma-Aldrich), and subsequently frozen at −80 °C. Frozen tissue was transferred to MagNA Lyser tubes (cat# 03358941001, Roche) containing 0.5 ml of RLT buffer supplemented with 1% β-mercaptoethanol, and homogenised with

(20) Association of α/β-Hydrolase D16B with Bovine Conception Rate and Sperm Plasma Membrane Lipid CompositionInternational journal of molecular sciencesJanuary 17, 2020Shan S, Xu F, Bleyer M, Becker S, Melbaum T, Wemheuer W, Hirschfeld M, Wacker C, Zhao S, Schütz E, et al.homogenization of tissue samples with MagNA lyser green beads (Roche Life Science, Mannheim, Product No. 03358941001, Germany) was carried out followed by an incubation for 2 h at 4 ◦ C and then centrifuged at 16,000×

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MagNA Lyser Green Beads from Roche

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MagNA Lyser Green Beads from Roche

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